Cell Culture Media
MSC SF Supplement
- Cat-No:CG0801
Volume: 25ml、50ml、100ml、500ml
Storage:≤-20°C
Origin: New Zealand
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Description
MSC SF Supplement is a fibrinogen depleted, non-xenogeneic (original material), animal serum-free supplement for replacing FBS (fetal bovine serum) to support cell expansion from research use.MSC SF Supplement contains abundant growth factors and cytokines necessary for research or industrial cell growth and proliferation of multiple cell types (e.g. MSCs) in research-scale production.
Due to a unique fibrinogen-depletion technique,MSC SF Supplement is a clot-free and heparin-free Serum Replacement Supplement. The product neither requires the addition of anticoagulants (e.g. heparin) in cell culture nor during manufacturing. That makes MSC SF Supplement a ready-to-use Serum Replacement Supplement that is widely applicable, since the absence of heparin offers even more convenient cell growth condition.
Instructions for Use
1. MSC SF Supplement shows optimal growth of MSC at 5% (v/v) in typical cell culture media, i.e. α-MEM, which contains 2mM L-Glutamine as final concentrate.
2. We recommend seeding MSCs at approximately 3x10 3~6x10 3 per cm2 .
3. MSC SF Supplement has been fibrinogen-depleted and does not require the addition of heparin in the cell culture media.
Important Information
1. Insoluble particles may form in thawed MSC SF Supplement. Published research has shown that particles will not alter the performance of the product.
2. Insoluble particles may form in thawed MSC SF Supplement, it is recommended to centrifuge at 3,400 xg for 3~5 minutes.
3. Filtering the completed medium (e.g. 5%), after MSC SF Supplement is diluted in the basal medium, will not affect MSC SF Supplement supplemented cell culture performance. However, 0.22 μ m filtering is NOT recommended for the 100% MSC SF Supplement concentrate, as this may reduce 5% MSC SF Supplement cell culture performance.
4. Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates and resulting potential decrease in MSC SF Supplement performance
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